Whole-Genome Amplification by Degenerate Oligonucleotide Primed PCR (DOP-PCR)
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چکیده
منابع مشابه
Whole-Genome Amplification by Degenerate Oligonucleotide Primed PCR (DOP-PCR).
INTRODUCTIONPCR-based whole-genome amplification (WGA) has the goal of generating microgram quantities of genome-representative DNA from picogram or nanogram amounts of starting material. This amplification should introduce little, or ideally no, representational bias. Unlike other techniques for WGA, PCR-based methods are generally less affected by DNA quality and are more applicable to DNA ex...
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Genetic alterations underlie the development and progression of neoplasia. Screening for genetic alterations in biopsies and premalignant lesions, where the neoplastic tissue analyzed usually constitutes only a small fraction of the cells in the specimen, often is difficult and tedious. Even after successful isolation of DNA, limited quantities allow only a few genetic analyses, limiting the id...
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Intrahepatic cholangiocarcinoma (ICC), a malignant neoplasm of the biliary epithelium,is usually fatal because of difficulty in early diagnosis and lack of availability of effective therapy. The genetic mechanisms involved in the development of ICC are not well understood and only a few cytogenetic studies of ICC have been published. Recently, technique of degenerate oligonucleotide primed (DOP...
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Random mutagenesis has become a powerful means of studying the effects of a large number of permutations of a particular DNA sequence. As a prime example, libraries of randomized cDNA clones, when translated into their corresponding proteins, can be useful in investigating the functional contributions of a mutagenized region to the overall properties of a protein. Existing molecular cloning tec...
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It is sometimes useful to amplify DNA before labeling it. Degenerate oligonucleotide primed-polymerase chain reaction (DOP-PCR) allows amplification of any complex DNA, regardless of sequence. DOP-PCR is performed using a single primer that has a fixed sequence at its 5' end and several degenerate bases near its 3' end that allow it to anneal at low stringency to many sites in complex DNA. Foll...
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ژورنال
عنوان ژورنال: Cold Spring Harbor Protocols
سال: 2008
ISSN: 1559-6095
DOI: 10.1101/pdb.prot4919